EBTT 2019

The meeting

Cell membrane electroporation — mechanisms & applications

Cell membrane electroporation, its mechanisms and applications in biology, medicine and biotechnology. The basis of the phenomena, current applications and future prospects were the main topics of this international meeting — the 10^th edition of the EBTT workshop and course.

The postgraduate course was intended both for novices and experts in electroporation, including PhD students, researchers and users. Abstract submission was strongly encouraged and was necessary if credits (5 ECTS) were sought; each participant submitting an abstract was also required to give a short oral presentation of the work. The course was accredited by the Medical Chamber of Slovenia, so participants received 34 CME credits.

Organised by the University of Ljubljana and the Institute of Oncology, Ljubljana.

Organising committee: Chair — Peter Kramar; Members — Matej Kranjc, Lea Vukanović, Eva Pirc, Duša Hodžić.

Ambassadors of the EBTT

Stefania Romero, PhD

CNR — Institute for Electromagnetic Sensing of the Environment (IREA), Napoli, Italy

Antoni Ivorra

Serra Húnter Fellow, Tenured Associate Professor, DTIC, Universitat Pompeu Fabra, Barcelona, Spain

Course content

Preliminary programme & topics

Electrical properties of cells & tissues

Resting & induced transmembrane voltage; dielectric properties; temperature & conductivity changes.

Physical chemistry of electroporation

Electrochemical membrane thermodynamics; molecular dynamics simulations.

Electroporation in vitro & in vivo

Protocols for cells in vitro and in vivo.

Devices & electrodes

Devices & electrodes; tissue models of electroporation in vivo and their validation.

Electrochemotherapy of tumours

Mechanisms, preclinical & clinical use, current developments & perspectives.

Gene transfection

Gene transfection in vitro/in vivo; clinical use in human medicine.

Transdermal drug delivery

Electroporation for gene delivery in the skin; DNA vaccines.

Highlights of 2019

Microfluidics for lab-on-chip (Cottet); PEF in food technology (Pataro); tumour ablation beyond the membrane (Govindarajan); PEF ablation for cardiac arrhythmias (Stewart).

Faculty

Directors & lecturers

Co-DirectorDamijan Miklavčič

Co-DirectorLluis M. Mir

Faculty members & lecturers

Damijan MiklavčičUniversity of Ljubljana, Slovenia
Lluis M. MirCNRS & Institut Gustave Roussy, Villejuif, France
Marie-Pierre RolsCNRS & IPBS, Toulouse, France
Gregor SeršaInstitute of Oncology, Ljubljana, Slovenia
Mounir TarekUniversity of Lorraine, Nancy, France
Justin TeissiéCNRS & IPBS, Toulouse, France
P. Thomas VernierOld Dominion University, Norfolk, Virginia, USA
Julie GehlUniversity of Copenhagen, Denmark

Invited lecturers

Jonathan CottetEPFL, Lausanne, Switzerland
Maja ČemažarInstitute of Oncology, Ljubljana, Slovenia
Srimathveeravalli GovindarajanMemorial Sloan-Kettering Cancer Center, New York, USA
Tadej KotnikUniversity of Ljubljana, Slovenia
Gianpiero PataroUniversity of Salerno, Italy
Mark StewartMedtronic, Minnesota, USA
Gaelle VandermeulenCatholic University of Louvain, Brussels, Belgium

Founding faculty members

Damijan MiklavčičUniversity of Ljubljana, Slovenia
Lluis M. MirCNRS & Institut Gustave Roussy, France
Eberhard NeumannUniversity of Bielefeld, Germany
Véronique PréatCatholic University of Louvain, Belgium
Gregor SeršaInstitute of Oncology, Ljubljana, Slovenia
Justin TeissiéCNRS & IPBS, Toulouse, France

Hands-on

Practical work & exercises

Wet-lab, computer-modelling, hardware and e-learning sessions. Exercise sheets (PDF) as distributed to participants, plus the laboratory-safety sheets.

S1	Laboratory safety	PDF ↓
S2	Electroporation hardware safety	PDF ↓
L1	The influence of Mg²⁺ ions on gene electrotransfer efficiency	PDF ↓
L2	Monitoring cell membrane electroporation with ratiometric fluorescent dye Fura-2AM	PDF ↓
L3	Visualisation of local ablation zone distribution between two needle electrodes	PDF ↓
L4	Effect of short high-frequency bipolar pulses on plasma membrane permeabilization	PDF ↓
L5	Electrotransformation of Escherichia coli with plasmid DNA	PDF ↓
L6	Electroporation of planar lipid bilayers	PDF ↓
L7	E. coli inactivation by pulsed electric fields in a continuous flow system	PDF ↓
L8	Analysis of electric field orientations on gene electrotransfer visualization at the membrane level	PDF ↓
L9	Comparison of flow cytometry and spectrofluorometric measurements in cell permeabilization experiments	PDF ↓
L10	Monitoring of electric field distribution in biological tissue by means of magnetic resonance electrical impedance tomography	PDF ↓
C1	Treatment planning for electrochemotherapy and irreversible electroporation: optimization of voltage & electrode position	PDF ↓
C2	Numerical modelling of thermal effects during irreversible electroporation treatments	PDF ↓
C3	Molecular dynamics simulations of membrane electroporation	PDF ↓
E1	Electroporation of cells and tissues — interactive e-learning	PDF ↓
H1	Measurement of electroporation pulses with oscilloscope, voltage and current probes	PDF ↓
H2	Development of pulsed power generators for electroporation	PDF ↓

Abstracts & publication

Abstract submission & proceedings

PDFInstructions for Authors PDFAbstract Submission Form DOCAbstract Template PDFJMB Author Guidelines

Participants were encouraged to submit a manuscript for a special issue of the Journal of Membrane Biology devoted to electroporation-based technologies and treatments.

Electronic proceedings — all abstracts and course material.

Download proceedings (PDF)

Contacts

Organisation & contacts

Registration, abstract submission & other

Damijan Miklavčič
Faculty of Electrical Engineering, University of Ljubljana
Tržaška 25, 1000 Ljubljana, Slovenia
damijan.miklavcic@fe.uni-lj.si
tel +386 1 4768 456 · fax +386 1 4264 658

Accommodation & travel

Congress Agency Albatros — Mrs. Nina Bernard
nina@albatros-bled.com
tel +386 4 5780 350 · fax +386 4 5780 355